[Main contributions] – The primary contributions of this work are (a) extending the SMC-EM technique to include the DH-PSF for three dimensional motion, (b) demonstrating the ability to do accurate localization and parameter estimation of confined motion at low signal and low SBR levels, and (c) providing a quantitative comparison to the standard method of GF-MSD and to other approaches based on optimal estimation across a range of (very low) SBR levels and confinement lengths [More …].
